Loading ...

J243. METHANOTHRIX MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.30 g 0.294 2.161
2 0.60 g 0.588 10.065
3 0.10 g 0.098 0.482
4 0.08 g 0.078 0.534
5 1.00 g 0.98 18.328
6 Trace element solution 10.00 ml - -
7 Trace vitamins 10.00 ml - -
8 6.80 g 6.667 81.267
9 4.00 g 3.922 39.171
10 0.50 g 0.49 2.791
11 0.50 g 0.49 2.041
12 1.00 mg 9.8e-4 0.004
13 1000.00 ml 978.634 54322.4
Instructions:
  1. Mix ingredients, except Trace vitamins, KHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, and adjust pH to 7.0. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20) and add KHCO3. Dispense the medium into suitable culture vessels (e.g., 50 ml of the medium in 120 ml serum bottles) under a stream of N2-CO2 (80:20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Filter-sterilize Trace vitamins under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add Trace vitamins, L-cysteine x HCl x H2O and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 100 kPa N2-CO2 (80:20).
Solution occurs in 5 other media (first defined in medium J243 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 12.800 g 12.8 66.967
2 1.350 g 1.35 -
3 0.100 g 0.1 -
4 0.024 g 0.024 -
5 0.100 g 0.1 0.68
6 0.100 g 0.1 0.734
7 0.025 g 0.025 -
8 0.010 g 0.01 0.162
9 1.000 g 1 17.111
10 0.120 g 0.12 -
11 0.026 g 0.026 -
12 1000.000 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
  2. Comment: For strain JCM 10134, incubate with a 10-20% inoculum.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Sodium acetate 6.667 81.267
KHCO3 3.922 39.171
NH4Cl 0.98 18.328
NaCl 0.598 10.233
Show more

Similar media

J807. WR MEDIUM FOR METHANOBACTERIACEAE 90 %
J272. JCM MEDIUM No. 272 89 %
J907. GEOBACTER MEDIUM 85 %
J881. KNH MEDIUM 83 %
J335. JCM MEDIUM No. 335 81 %

Latest comments

No comments for this medium.