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J251. METHANOTHERMUS FERVIDUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Mineral solution I 37.5 ml - -
2 Mineral solution II 37.5 ml - -
3 3.0 mg 0.003 -
4 2.0 mg 0.002 0.007
5
(BD-Difco)
2.0 g 1.97 -
6
(BD-BBL)
2.0 g 1.97 -
7 Trace vitamins 10.0 ml - -
8 Trace minerals 10.0 ml - -
9 3.4 g 3.35 23.583
10 2.0 g 1.97 23.456
11 1.0 mg 9.9e-4 0.004
12 0.5 g 0.493 2.051
13 0.5 g 0.493 2.805
14 920.0 ml 904.779 50222.8
Instructions:
  1. Mix ingredients, except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20), and add NaHCO3 to the medium. Dispense the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) gas mixture and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight and adjust pH to 6.5, if necessary. Separately autoclave L-cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the L-cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 6 g 6 34.448
2 1000 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 6.0 g 6 44.09
2 6.0 g 6 45.406
3 12.0 g 12 205.33
4 2.4 g 2.4 9.737
5 1.6 g 1.6 10.884
6 1000.0 ml 998.207 55408.8
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Na2SO4 3.35 23.583
NaHCO3 1.97 23.456
Yeast extract 1.97 -
Trypticase peptone 1.97 -
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