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JCM 268 . MJ/YPGS MEDIUM

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD-Difco)
1.00 g 1 -
Bacto peptone
(BD-Difco)
1.00 g 1 -
Glucose 0.20 g 0.2 1.11
Sodium succinate 0.20 g 0.2 1.234
Na2SiO3 x 9 H2O 1.00 g 1 -
Na2S2O3 x 5 H2O 2.48 g 2.48 9.993
MJ(-N) synthetic seawater 100.00 ml - -
Distilled water 900.00 ml - -
1 Adjust pH to 7.5.
Solution occurs in 12 other media (first defined in Medium J268).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.703 508.264
K2HPO4 0.14 g 0.139 0.796
CaCl2 x 2 H2O 0.14 g 0.139 0.943
MgSO4 x 7 H2O 3.40 g 3.366 13.658
MgCl2 x 6 H2O 4.18 g 4.139 20.357
KCl 0.33 g 0.327 4.383
NiCl2 x 6 H2O 0.50 mg 5.0e-4 0.004
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 0.01 -
Modified Wolin's mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 2.971 50.843
Na2S2O3 x 5 H2O 2.48 9.993
Yeast extract 1 -
Bacto peptone 1 -
Na2SiO3 x 9 H2O 1 -
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