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J272. JCM MEDIUM No. 272

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.40 g 0.392 2.882
2 0.90 g 0.882 16.495
3 0.36 g 0.353 1.736
4 10.00 g 9.804 167.753
5 0.50 g 0.49 5.976
6
(BD-Difco)
2.00 g 1.961 -
7
(BD-BBL)
2.00 g 1.961 -
8 Trace vitamins 10.00 ml - -
9 Trace element solution 10.00 ml - -
10 5.00 g 4.902 46.25
11 0.50 g 0.49 2.791
12 0.50 g 0.49 2.041
13 1.00 mg 9.8e-4 0.004
14 1000.00 ml 978.634 54322.4
Instructions:
  1. Mix ingredients, except Na2CO3, L-cysteine x HCl x H2O and Na2S x 9H2O. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v) and add Na2CO3. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the L-cysteine x HCl x H2O and Na2S x 9H2O solutions. Check pH of the medium to be around 7.5. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).
Solution occurs in 5 other media (first defined in medium J243 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 12.800 g 12.8 66.967
2 1.350 g 1.35 -
3 0.100 g 0.1 -
4 0.024 g 0.024 -
5 0.100 g 0.1 0.68
6 0.100 g 0.1 0.734
7 0.025 g 0.025 -
8 0.010 g 0.01 0.162
9 1.000 g 1 17.111
10 0.120 g 0.12 -
11 0.026 g 0.026 -
12 1000.000 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
  2. Comment: For strain JCM 10134, incubate with a 10-20% inoculum.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 9.814 167.921
Na2CO3 4.902 46.25
Yeast extract 1.961 -
Trypticase peptone 1.961 -
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