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JCM 273 . TM BROTH MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J273 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Polypeptone
(Nihon Pharm. Co.) 		4 g 3.96 -
Yeast extract
(BD-Difco) 		2 g 1.98 -
NaCl 1 g 0.99 16.942
Castenholz basal salt solution 10 ml - -
Distilled water 1000 ml - -
1 Adjust pH to 7.2.

Castenholz basal salt solution #

1020
Solution occurs in 2 other media (first defined in Medium J273).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.00 g 0.98 5.129
CaSO4 x 2 H2O 0.60 g 0.588 3.417
MgSO4 x 7 H2O 1.00 g 0.98 3.978
NaCl 0.08 g 0.078 1.342
KNO3 1.03 g 1.01 9.988
NaNO3 6.89 g 6.755 79.474
Na2HPO4 1.11 g 1.088 7.666
FeCl3 x 6 H2O
(0.03%) 		10.00 ml - -
Nitsch's trace elements 10.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 8.2.

Nitsch's trace elements #

1000
Solution occurs in 1 other media (first defined in Medium J273).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2SO4 0.500 ml - -
MnSO4 x n H2O 2.200 g 2.2 -
ZnSO4 x 7 H2O 0.500 g 0.5 1.739
H3BO3 0.500 g 0.5 8.085
CuSO4 x 5 H2O 0.016 g 0.016 0.064
Na2MoO4 x 2 H2O 0.025 g 0.025 0.103
CoCl2 x 6 H2O 0.046 g 0.046 0.354
Distilled water 1000.000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.2
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Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Polypeptone 3.96 -
Yeast extract 1.98 -
NaCl 0.991 16.955
NaNO3 0.067 0.787
Na2HPO4 0.011 0.076
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen facultative anaerobe 1 strain
temperature thermophilic 4 strains