JCM 276 . CASTENHOLZ MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tryptone
(BD-Difco)
1 g 1 14.069
Yeast extract
(BD-Difco)
1 g 1 -
Castenholz basal salt solution 100 ml - -
Distilled water 900 ml - -
1 Adjust pH to 8.2 with NaOH.
Solution occurs in 2 other media (first defined in Medium J273).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.00 g 0.98 5.129
CaSO4 x 2 H2O 0.60 g 0.588 3.417
MgSO4 x 7 H2O 1.00 g 0.98 3.978
NaCl 0.08 g 0.078 1.342
KNO3 1.03 g 1.01 9.988
NaNO3 6.89 g 6.755 79.474
Na2HPO4 1.11 g 1.088 7.666
FeCl3 x 6 H2O
(0.03%)
10.00 ml - -
Nitsch's trace elements 10.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 8.2.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.2
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Associated strains

All strains for this medium: 16

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 1 14.069
Yeast extract 1 -
NaNO3 0.675 7.947
Na2HPO4 0.109 0.767
KNO3 0.101 0.999
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Cultivation metadata from 16 strains BacDive

pH 8 - 10 2 strains
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 16 strains
oxygen aerobe 2 strains
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