J278
278 .
NAM AGAR
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J278 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase soy agar
(BD-BBL) |
40 | g | 38.095 | - | |
|
Defibrinated sheep blood
(5%) |
50 | ml | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 After preparing the Trypticase soy agar plate with 5%sheep blood, spreading the subculture on the agar surface and place one NAM disk aseptically. Incubate the agar plate under a H2-CO2-N2 (1:1:8) gas mixture. To prepare the NAM disk, impregnate with 20 �l filter-sterilized 1.5% NAM (N-acetylmuramic acid, Sigma) aqueous solution to a sterile paper disk (0.64 cm in diameter). Stored the air-dried disk at room temperature. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase soy agar | 38.095 | - |
| Defibrinated sheep blood | - | - |
| Distilled water | - | - |
