Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgSO4 x 7 H2O | 7.00 | g | 6.931 | 28.119 | |
MgCl2 x 6 H2O | 5.50 | g | 5.446 | 26.785 | |
KCl | 0.65 | g | 0.644 | 8.633 | |
NaBr | 0.10 | g | 0.099 | 0.962 | |
NaHCO3 | 2.00 | g | 1.98 | 23.572 | |
NH4Cl | 0.15 | g | 0.149 | 2.777 | |
CaCl2 x 2 H2O | 0.50 | g | 0.495 | 3.367 | |
Trace minerals | 10.00 | ml | - | - | |
(NH4)2Ni(SO4)2 x 6 H2O | 0.02 | g | 0.02 | 0.05 | |
Na2WO4 x 2 H2O | 0.10 | mg | 9.9e-5 | 3.0e-4 | |
Na2SeO4 | 0.10 | mg | 9.9e-5 | 5.2e-4 | |
Na2S2O3 x 5 H2O | 1.00 | g | 0.99 | 3.989 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 7.0 with H2SO4. Distribute 20 ml of medium into 120 ml serum bottles under a stream of a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Autoclave and stand the bottles overnight. Add 15 ml filter-sterilized air and pressurize the gas phase to 200 kPa with a H2-CO2 (4:1, v/v) gas mixture. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MgSO4 x 7 H2O | 6.96 | 28.24 |
MgCl2 x 6 H2O | 5.446 | 26.785 |
NaHCO3 | 1.98 | 23.572 |
Na2S2O3 x 5 H2O | 0.99 | 3.989 |
KCl | 0.644 | 8.633 |
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