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JCM 284 . SI MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

904
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD-Difco) 		0.10 g 0.111 -
KH2PO4 0.14 g 0.155 1.138
MgCl2 x 6 H2O 0.20 g 0.221 1.088
CaCl2 x 2 H2O 0.15 g 0.166 1.129
NH4Cl 0.54 g 0.597 11.167
Trace vitamins solution 2.00 ml - -
Trace element solution 1.00 ml - -
Se/W solution 1.00 ml - -
NaHCO3 2.50 g 2.765 32.92
Resazurin 1.00 mg 0.001 0.005
Distilled water 900.00 ml - -
1 Mix components except NaHCO3 and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense 0.9 volume of the medium into culture vessels (e.g., 18 ml of the medium in 50 ml serum bottle) under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight.

Solution B #

100
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium pyruvate 2.2 g 22 199.927
Distilled water 100.0 ml - -
1 Adjust pH to 7.0 and filter-sterilize. Keep the solution under a N2 atmosphere.
2 To complete the medium, add 0.1 volume of Solution B to the culture vessels containing Solution A. Prior to inoculation, aseptically and anaerobically add 0.01 volume of L-cysteine x HCl x H2O and Na2S x 9H2O solutions to the medium (3% solutions, autoclaved and stored under a N2 atmosphere).
3 Comment: For strain JCM 10971, incubate with a 10% inoculum.

Trace vitamins solution #

1000
Solution occurs in 9 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.9 mg 0.005 0.02
Folic acid 8.8 mg 0.009 0.02
Pyridoxine hydrochloride 4.1 mg 0.004 -
Thiamine HCl 6.7 mg 0.007 -
Riboflavin 7.5 mg 0.008 0.02
Nicotinic acid 2.5 mg 0.003 0.02
DL-Calcium pantothenate 9.5 mg 0.01 0.02
Vitamin B12 27.1 mg 0.027 0.02
p-Aminobenzoic acid 2.7 mg 0.003 0.02
Lipoic acid 4.1 mg 0.004 0.02
Distilled water 1000.0 ml - -

Trace element solution #

1000
Solution occurs in 7 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH.

Se/W solution #

1000
Solution occurs in 8 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 4 mg 0.004 0.015
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain