Name: HALORHABDUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J294 #

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	270.000	g	270	4620.12
NaBr	0.100	g	0.1	0.972
MgSO₄ x 7 H₂O	20.000	g	20	81.144
KCl	5.000	g	5	67.069
NH₄Cl	2.000	g	2	37.39
Tris-HCl buffer	12.000	g	12	-
Yeast extract (BD-Difco)	1.000	g	1	-
Trace metal solution	2.000	ml	-	-
Glucose	2.000	g	2	11.101
KH₂PO₄	0.125	g	0.125	0.919
CaCl₂ x 2 H₂O	0.050	g	0.05	0.34
FeCl₂ x 4 H₂O	5.000	mg	0.005	0.025
MnCl₂ x 4 H₂O	5.000	mg	0.005	0.025
1 Add components except yeast extract to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with NaOH and sterilize by filtration. Autoclave yeast extract as a 10% (w/v) solution and aseptically add to the mineral solution.

Trace metal solution #

1010

Solution occurs in 3 other media (first defined in Medium J294).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (32%)	10.000	ml	3.168	86.898
FeCl₂ x 4 H₂O	2.000	g	1.98	9.96
CoCl₂ x 6 H₂O	0.250	g	0.248	1.906
MnCl₂ x 4 H₂O	0.100	g	0.099	0.5
ZnCl₂	0.070	g	0.069	0.508
H₃BO₃	6.000	mg	0.006	0.096
Na₂MoO₄ x 2 H₂O	0.040	g	0.04	0.164
NiCl₂ x 6 H₂O	0.070	g	0.069	0.535
CuCl₂ x 2 H₂O	2.000	mg	0.002	0.012
AlCl3	0.025	g	0.025	0.186
Na₂WO₄ x 2 H₂O	6.000	mg	0.006	0.018
Distilled water	1000.000	ml	-	-

All strains for this medium:

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