J301
301 .
THERMODESULFOBACTERIUM HYDROGENOPHILUM MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J301 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 29.644 | 507.26 | |
| MgCl2 x 6 H2O | 3.00 | g | 2.964 | 14.581 | |
| Na2SO4 | 4.00 | g | 3.953 | 27.827 | |
| KCl | 0.50 | g | 0.494 | 6.627 | |
| NH4Cl | 0.25 | g | 0.247 | 4.618 | |
| KH2PO4 | 0.20 | g | 0.198 | 1.452 | |
| PIPES | 3.46 | g | 3.419 | 11.306 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.148 | 1.008 | |
|
Yeast extract
(BD-Difco) |
0.50 | g | 0.494 | - | |
| Sodium acetate | 0.83 | g | 0.82 | 9.998 | |
| Trace element solution | 1.00 | ml | - | - | |
| Na2WO4 x 2 H2O | 0.20 | mg | 2.0e-4 | 6.0e-4 | |
| Na2SeO4 | 0.05 | mg | 4.9e-5 | 2.6e-4 | |
| Trace vitamins | 10.00 | ml | - | - | |
| Vitamin B12 | 0.05 | mg | 4.9e-5 | 3.6e-5 | |
| Growth stimulating factors | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Na2S x 9 H2O | 0.50 | g | 0.494 | 2.057 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients, except Na2S x 9H2O, and adjust pH to 6.5 with HCl. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers, and autoclave. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Add the sterile Na2S solution to the medium, and pressurize the bottles to 200 kPa H2-CO2 (4:1, v/v). After incubation at 70C for 1 hr, readjust pH to 6.5 with a sterile and anaerobic NaHCO3 stock solution. | |||||
Solution occurs in 5
other media (first defined in Medium J301).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 12.80 | g | - | - | |
| FeSO4 x 7 H2O | 0.42 | g | - | - | |
| H3BO3 | 0.01 | g | - | - | |
| MnCl2 x 4 H2O | 0.10 | g | - | - | |
| CoCl2 x 6 H2O | 0.17 | g | - | - | |
| CuCl2 x 2 H2O | 0.02 | g | - | - | |
| ZnSO4 x 7 H2O | 0.21 | g | - | - | |
| Na2MoO4 x 2 H2O | 0.01 | g | - | - | |
| 1 Dissolve nitrilotriacetic acid in 800 ml distilled water and adjust pH to 6.5 with NaOH. Then proceed to add the minerals and bring volume to 1.0 L. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Isobutyric acid | 0.5 | g | 5 | 56.747 | |
| Valeric acid | 0.5 | g | 5 | 48.957 | |
| 2-Methylbutyric acid | 0.5 | g | 5 | - | |
| 3-Methylbutyric acid | 0.5 | g | 5 | - | |
| Caproic acid | 0.2 | g | 2 | 17.218 | |
| Succinic acid | 0.6 | g | 6 | 50.809 | |
| Distilled water | 100.0 | ml | - | - | |
| 1 Adjust pH to 9.0 with NaOH. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 9.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |