J306
306 .
HALOALKALIPHILE MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD-Difco) |
2.0 | g | - | - | |
|
Casamino acids
(BD-Difco) |
2.0 | g | - | - | |
| Na glutamate | 1.0 | g | - | - | |
| Trisodium citrate | 3.0 | g | - | - | |
| MgSO4 x 7 H2O | 0.1 | g | - | - | |
| CaSO4 x 2 H2O | 0.1 | g | - | - | |
| KCl | 1.0 | g | - | - | |
| NaCl | 200.0 | g | - | - | |
| Na2CO3 | 8.0 | g | - | - | |
| 1 Add components except Na2CO3 to distilled water and bring volume to 900 ml. Adjust pH to 6.5 and autoclave. Dissolve Na2CO3 in 100 ml distilled water and autoclave separately. Aseptically add the sterile Na2CO3 solution to the medium. Check final pH to be 9.0 - 9.5. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 11 |
