MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 308 . ACIDILOBUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J308 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.326 6.096
KH2PO4 0.33 g 0.326 2.396
KCl 0.33 g 0.326 4.374
CaCl2 x 2 H2O 0.33 g 0.326 2.218
MgCl2 x 6 H2O 0.33 g 0.326 1.604
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Trace vitamins 10.00 ml - -
Yeast extract
(BD-Difco) 		0.30 g 0.296 -
Sulfur
(powder) 		10.00 g 9.881 308.12
Na2S x 9 H2O 0.50 g 0.494 2.057
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients, except yeast extract, vitamins, sulfur and Na2S x 9H2O, and autoclave under a N2-CO2 (80:20) gas atmosphere. Separately autoclave 10% (w/v) yeast extract solution and 5% (w/v) Na2S x 9H2O solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize Trace vitamins solution while gassing with N2. Aseptically and anaerobically add the yeast extract and vitamin solutions, and sulfur to the medium under a N2-CO2 (80:20) gas atmosphere. Prior to inoculation, add sterile Na2S x 9H2O solution, and adjust pH to 3.5 - 3.8 with sterile 1 N H2SO4. Pressurize the inoculated bottle to 100 kPa N2-CO2 (80:20).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 3.6
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 9.881 308.12
Na2S x 9 H2O 0.494 2.057
NH4Cl 0.326 6.096
KCl 0.326 4.374
KH2PO4 0.326 2.396
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain