MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 315 . JCM MEDIUM No. 315

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J315 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.78 g 0.765 4.39
KH2PO4 0.75 g 0.735 5.403
Na3-EDTA 0.04 g 0.039 -
FeSO4 x 7 H2O 0.01 g 0.01 0.035
MgSO4 x 7 H2O 0.25 g 0.245 0.994
CaCl2 x 2 H2O 0.03 g 0.029 0.2
NaCl 0.20 g 0.196 3.355
NH4Cl 0.50 g 0.49 9.164
Glucose 0.50 g 0.49 2.721
Yeast extract
(BD-Difco) 		0.50 g 0.49 -
Na2S2O3 x 5 H2O 1.24 g 1.216 4.898
Trace vitamins 10.00 ml - -
Trace mineral solution 10.00 ml - -
NaHCO3 5.00 g 4.902 58.352
L-Cysteine HCl x H2O 0.50 g 0.49 -
Distilled water 1000.00 ml - -
1 Mix ingredients except cysteine x HCl x H2O, NaHCO3 and Trace vitamins, and autoclave under a N2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as a 10% solution and Trace vitamins while gassing with CO2. Separately autoclave L-cysteine x HCl x H2O as a 5% solution under a CO2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile L-cysteine x HCl x H2O solution.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace mineral solution #

1000
Solution occurs in 2 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 4.500 g 4.5 23.543
FeCl2 x 4 H2O 0.400 g 0.4 2.012
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.170 g 0.17 1.309
ZnCl2 0.100 g 0.1 0.734
CaCl2 x 2 H2O 0.020 g 0.02 0.136
H3BO3 0.019 g 0.019 0.307
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.030 g 0.03 0.231
Distilled water 1000.000 ml - -
1 Adjust pH to 7.0 with 1 N KOH.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.902 58.352
Na2S2O3 x 5 H2O 1.216 4.898
K2HPO4 0.765 4.39
KH2PO4 0.735 5.403
NH4Cl 0.49 9.164
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain