Name: THERMOCRINIS ALBUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J316 #

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	256.0	mg	0.253	4.337
KH₂PO₄	1.7	mg	0.002	0.012
CaCl₂ x 2 H₂O	0.8	mg	7.9e-4	0.005
H₃BO₃	10.3	mg	0.01	0.165
Na₂SO₄	23.0	mg	0.023	0.16
NaNO₃	0.3	mg	3.0e-4	0.003
KCl	15.0	mg	0.015	0.199
FeCl₃ x 6 H₂O	0.1	mg	9.9e-5	3.7e-4
MnSO₄ x n H₂O	6.0	µg	5.9e-6	-
Trace minerals	10.0	ml	-	-
(NH₄)₂Ni(SO₄)₂ x 6 H₂O	2.0	mg	0.002	0.005
Na₂WO₄ x 2 H₂O	0.1	mg	9.9e-5	3.0e-4
Na₂SeO₄	0.1	mg	9.9e-5	5.2e-4
NaHCO₃	1.0	g	0.99	11.786
Na₂S₂O₃ x 5 H₂O	1.0	g	0.99	3.989
Distilled water	1000.0	ml	-	-
1 Mix ingredients except Na₂S₂O₃ x 5H₂O, distribute the medium to culture vessels under a N₂ gas atmosphere, and seal with butyl rubber stoppers. Autoclave and stand the culture vessels overnight. Add filter-sterilized 10% Na₂S₂O₃ x 5H₂O solution and adjust pH to 7.0 with 1 N H₂SO₄. Replace the gas phase with a N₂-O₂-H₂ (96:1:3, v/v) gas mixture, and pressurized the inoculated bottles to 300 kPa.

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

All strains for this medium:

CSV

JSON