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JCM 317 . JCM MEDIUM No. 317

Culture medium recipe

Vol.
×

Main sol. J317 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 2.20 g 2.191 12.163
Yeast extract
(BD-Difco) 		2.30 g 2.291 -
KH2PO4 0.14 g 0.139 1.025
MgCl2 x 6 H2O 0.20 g 0.199 0.98
CaCl2 x 2 H2O 0.15 g 0.149 1.016
NH4Cl 0.54 g 0.538 10.055
NaHCO3 2.50 g 2.49 29.641
Na2S x 9 H2O 0.30 g 0.299 -
L-Cysteine HCl x H2O 0.30 g 0.299 -
Trace vitamins 2.00 ml - -
Trace element solution 1.00 ml - -
Se/W solution 1.00 ml - -
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -

Trace element solution #

1000
Solution occurs in 7 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH.

Se/W solution #

1000
Solution occurs in 4 other media (first defined in Medium J317).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 4 mg 0.004 -
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: n.d.

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.49 29.641
Yeast extract 2.291 -
Glucose 2.191 12.163
NH4Cl 0.538 10.055
Na2S x 9 H2O 0.299 -
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