317 .
JCM MEDIUM No. 317
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Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Glucose | 2.20 | g |
|
|
Yeast extract
(BD-Difco) |
2.30 | g |
|
|
KH2PO4 | 0.14 | g |
|
|
MgCl2 x 6 H2O | 0.20 | g |
|
|
CaCl2 x 2 H2O | 0.15 | g |
|
|
NH4Cl | 0.54 | g |
|
|
NaHCO3 | 2.50 | g |
|
|
Na2S x 9 H2O | 0.30 | g |
|
|
L-Cysteine HCl x H2O | 0.30 | g |
|
|
Trace vitamins | 2.00 | ml |
|
|
Trace element solution | 1.00 | ml |
|
|
Se/W solution | 1.00 | ml |
|
|
Resazurin | 1.00 | mg |
|
|
Distilled water | 1000.00 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 12.800 | g | ||
|
|
FeCl3 x 6 H2O | 1.350 | g | ||
|
|
MnCl2 x 4 H2O | 0.100 | g | ||
|
|
CoCl2 x 6 H2O | 0.024 | g | ||
|
|
CaCl2 x 2 H2O | 0.100 | g | ||
|
|
ZnCl2 | 0.100 | g | ||
|
|
CuCl2 x 2 H2O | 0.025 | g | ||
|
|
H3BO3 | 0.010 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.024 | g | ||
|
|
NiCl2 x 6 H2O | 0.120 | g | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH. | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |