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JCM 324 . MMJY MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J324 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.412 503.281
K2HPO4 0.14 g 0.137 0.788
CaCl2 x 2 H2O 1.00 g 0.98 6.669
NaNO3 0.80 g 0.784 9.228
MgSO4 x 7 H2O 3.40 g 3.333 13.524
MgCl2 x 6 H2O 4.18 g 4.098 20.157
KCl 0.33 g 0.324 4.34
NiCl2 x 6 H2O 5.00 mg 0.005 0.038
Na2SeO3 1.00 mg 9.8e-4 -
Fe2(SO4)3 x n H2O 0.05 g 0.049 -
H2WO4 5.00 mg 0.005 0.02
Trace mineral solution 10.00 ml - -
Trace vitamins 10.00 ml - -
NaHCO3 1.00 g 0.98 11.67
Yeast extract 1.00 g 0.98 -
Sodium acetate 0.40 g 0.392 4.781
Sulfur
(powder) 		30.00 g 29.412 917.111
Na2S x 9 H2O 0.50 g 0.49 2.041
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients except Trace vitamins, NaHCO3, sulfur and Na2S x 9H2O, adjust pH to 7.5 - 8.0 and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.6 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the sterile Na2S solution. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).

Trace mineral solution #

1000
Solution occurs in 2 other media (first defined in Medium J324).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x n H2O 0.50 g 0.5 -
CoSO4 x 7 H2O 0.50 g 0.5 3.226
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.02 g 0.02 0.077
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 29.412 917.111
NaCl 29.412 503.281
MgCl2 x 6 H2O 4.098 20.157
MgSO4 x 7 H2O 3.333 13.524
NaHCO3 0.98 11.67
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
nutrition heterotroph 1 strain
oxygen anaerobe 1 strain
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