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J325. MJ/ANHOX-NO3 WITH THIOSULFATE MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.05 g 0.05 0.934
2 0.60 g 0.599 -
3 0.85 g 0.849 9.991
4 2.48 g 2.478 -
5 2.00 g 1.998 23.784
6 Trace vitamins 1.00 ml - -
7 MJ(-N) synthetic seawater 100.00 ml - -
8 900.00 ml 897.489 49818.1
Instructions:
  1. Distribute medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers, and autoclave. Adjust pH to 7.5, if necessary. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 10 other media (first defined in medium J268 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 29.703 508.243
2 0.14 g 0.139 0.796
3 0.14 g 0.139 0.943
4 3.40 g 3.366 13.658
5 4.18 g 4.139 20.357
6 0.33 g 0.327 4.383
7 0.50 mg 5.0e-4 -
8 0.50 mg 5.0e-4 -
9 0.01 g 0.01 -
10 Trace minerals 10.00 ml - -
11 1000.00 ml 988.324 54860.2

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 2.968 50.791
Na2S2O3 x 5H2O 2.478 -
NaHCO3 1.998 23.784
NaNO3 0.849 9.991
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