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J335. JCM MEDIUM No. 335

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.78 g 0.765 4.39
2 0.75 g 0.735 5.403
3 0.04 g 0.039 -
4 0.01 g 0.01 -
5 0.25 g 0.245 -
6 0.03 g 0.029 0.228
7 0.20 g 0.196 3.355
8 27.00 mg 0.026 0.495
9 2.80 g 2.745 -
10 0.16 g 0.157 1.912
11 Trace vitamins 10.00 ml - -
12 Trace element solution 10.00 ml - -
13 0.25 g 0.245 1.395
14 1000.00 ml 978.634 54322.4
Instructions:
  1. Adjust pH to 5.5. Fill the gas phase with H2-CO2 (80:20).
Solution occurs in 5 other media (first defined in medium J243 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 12.800 g 12.8 66.967
2 1.350 g 1.35 -
3 0.100 g 0.1 -
4 0.024 g 0.024 -
5 0.100 g 0.1 0.68
6 0.100 g 0.1 0.734
7 0.025 g 0.025 -
8 0.010 g 0.01 0.162
9 1.000 g 1 17.111
10 0.120 g 0.12 -
11 0.026 g 0.026 -
12 1000.000 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
  2. Comment: For strain JCM 10134, incubate with a 10-20% inoculum.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaSO4 2.745 -
K2HPO4 0.765 4.39
KH2PO4 0.735 5.403
L-Cysteine x HCl x H2O 0.245 1.395
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J881. KNH MEDIUM 74 %

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