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JCM 339 . SD0 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J339 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 100.00 g 98.912 1692.54
MgSO4 x 7 H2O 3.45 g 3.412 13.845
MgCl2 x 6 H2O 3.00 g 2.967 14.596
NH4Cl 0.25 g 0.247 4.623
CaCl2 x 2 H2O 0.14 g 0.138 0.942
CaSO4 x 2 H2O 0.25 g 0.247 1.436
KH2PO4 0.14 g 0.138 1.018
NaHCO3 1.00 g 0.989 11.774
Trace minerals 1.00 ml - -
Trace vitamins 10.00 ml - -
Yeast extract
(BD-Difco) 		0.10 g 0.099 -
Distilled water 1000.00 ml - -
1 Adjust pH to 6.0 with HCl. Sterilize the medium by filtration.

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 98.913 1692.56
MgSO4 x 7 H2O 3.415 13.857
MgCl2 x 6 H2O 2.967 14.596
NaHCO3 0.989 11.774
NH4Cl 0.247 4.623
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain