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J339. SD0 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100.00 g 98.912 1692.47
2 3.45 g 3.412 13.845
3 3.00 g 2.967 14.596
4 0.25 g 0.247 4.623
5 0.14 g 0.138 0.942
6 0.25 g 0.247 -
7 0.14 g 0.138 1.018
8 1.00 g 0.989 11.774
9 Trace minerals 1.00 ml - -
10 Trace vitamins 10.00 ml - -
11
(BD-Difco)
0.10 g 0.099 -
12 1000.00 ml 987.346 54806
Instructions:
  1. Adjust pH to 6.0 with HCl. Sterilize the medium by filtration.
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
NaCl 98.913 1692.49
MgSO4 x 7H2O 3.415 13.857
MgCl2 x 6H2O 2.967 14.596
NaHCO3 0.989 11.774
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