Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MJ(-N) synthetic seawater | 670.00 | ml | - | - | |
NH4Cl | 0.25 | g | 0.245 | 4.573 | |
NaNO3 | 1.00 | g | 0.978 | 11.512 | |
Na2WO4 x 2 H2O | 0.10 | mg | 9.8e-5 | - | |
Distilled water | 330.00 | ml | - | - | |
1 Mix components thoroughly, adjust pH to 7.0 with NaOH and autoclave. After cooling, add the following solutions (filter-sterilized): | |||||
NaHCO3
(8%) |
12.50 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
2 Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize inoculated bottles to 200 kPa H2-CO2 (4:1, v/v). | |||||
3 Comment: For JCM 34026, use 1.0 L of MJ(-N) synthetic seawater and omit distilled water. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 30.00 | g | 29.703 | 508.264 |
K2HPO4 | 0.14 | g | 0.139 | 0.796 |
CaCl2 x 2 H2O | 0.14 | g | 0.139 | 0.943 |
MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 |
MgCl2 x 6 H2O | 4.18 | g | 4.139 | 20.357 |
KCl | 0.33 | g | 0.327 | 4.383 |
NiCl2 x 6 H2O | 0.50 | mg | 5.0e-4 | 0.004 |
Na2SeO3 x 5 H2O | 0.50 | mg | 5.0e-4 | 0.002 |
Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - |
Modified Wolin's mineral solution | 10.00 | ml | - | - |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 19.479 | 333.317 |
MgCl2 x 6 H2O | 2.713 | 13.346 |
MgSO4 x 7 H2O | 2.226 | 9.033 |
NaNO3 | 0.978 | 11.512 |
NH4Cl | 0.245 | 4.573 |
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