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JCM 344 . HNW MEDIUM

Culture medium recipe

Vol.
×
Solution occurs in 1 other media (first defined in Medium J344).
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MJ(-N) synthetic seawater 670.00 ml - -
NH4Cl 0.25 g 0.245 4.573
NaNO3 1.00 g 0.978 11.512
Na2WO4 x 2 H2O 0.10 mg 9.8e-5 -
Distilled water 330.00 ml - -
1 Mix components thoroughly, adjust pH to 7.0 with NaOH and autoclave. After cooling, add the following solutions (filter-sterilized):
NaHCO3
(8%)
12.50 ml - -
Trace vitamins 10.00 ml - -
2 Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
3 Comment: For JCM 34026, use 1.0 L of MJ(-N) synthetic seawater and omit distilled water.
Solution occurs in 12 other media (first defined in Medium J268).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.703 508.264
K2HPO4 0.14 g 0.139 0.796
CaCl2 x 2 H2O 0.14 g 0.139 0.943
MgSO4 x 7 H2O 3.40 g 3.366 13.658
MgCl2 x 6 H2O 4.18 g 4.139 20.357
KCl 0.33 g 0.327 4.383
NiCl2 x 6 H2O 0.50 mg 5.0e-4 0.004
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 0.01 -
Modified Wolin's mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.479 333.317
MgCl2 x 6 H2O 2.713 13.346
MgSO4 x 7 H2O 2.226 9.033
NaNO3 0.978 11.512
NH4Cl 0.245 4.573
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Cultivation metadata from 2 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain

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