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JCM 344 . HNW MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J344 #

ml
Solution occurs in 1 other media (first defined in Medium J344).
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MJ(-N) synthetic seawater 670.00 ml - -
NH4Cl 0.25 g 0.245 4.573
NaNO3 1.00 g 0.978 11.512
Na2WO4 x 2 H2O 0.10 mg 9.8e-5 -
Distilled water 330.00 ml - -
1 Mix components thoroughly, adjust pH to 7.0 with NaOH and autoclave. After cooling, add the following solutions (filter-sterilized):
NaHCO3
(8%) 		12.50 ml - -
Trace vitamins 10.00 ml - -
2 Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
3 Comment: For JCM 34026, use 1.0 L of MJ(-N) synthetic seawater and omit distilled water.

MJ(-N) synthetic seawater #

1010
Solution occurs in 12 other media (first defined in Medium J268).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.703 508.264
K2HPO4 0.14 g 0.139 0.796
CaCl2 x 2 H2O 0.14 g 0.139 0.943
MgSO4 x 7 H2O 3.40 g 3.366 13.658
MgCl2 x 6 H2O 4.18 g 4.139 20.357
KCl 0.33 g 0.327 4.383
NiCl2 x 6 H2O 0.50 mg 5.0e-4 0.004
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 0.01 -
Modified Wolin's mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.479 333.317
MgCl2 x 6 H2O 2.713 13.346
MgSO4 x 7 H2O 2.226 9.033
NaNO3 0.978 11.512
NH4Cl 0.245 4.573
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 2 strains
nutrition chemolithoautotroph 1 strain
oxygen microaerophile 1 strain
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