Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Synthetic seawater (2 x)
(2 x) |
500.0 | ml | - | - | |
KH2PO4 | 1.0 | g | 1 | 7.348 | |
(NH4)2SO4 | 0.5 | g | 0.5 | 3.784 | |
Yeast extract
(BD-Difco) |
0.4 | g | 0.4 | - | |
Sulfur
(powder) |
10.0 | g | 10 | 311.818 | |
Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
Distilled water | 500.0 | ml | - | - | |
1 Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, and autoclave. Separately autoclave 10% (w/v) yeast extract solution, and 5% Na2S x 9H2O solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sulfur under a stream of H2-CO2 (4:1, v/v) gas mixture, add the yeast extract solution, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v) gas mixture. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 55.40 | g | 55.4 | 947.981 |
MgSO4 x 7 H2O | 14.00 | g | 14 | 56.801 |
MgCl2 x 6 H2O | 11.00 | g | 11 | 54.106 |
KCl | 1.30 | g | 1.3 | 17.438 |
NaBr | 0.20 | g | 0.2 | 1.944 |
H3BO3 | 0.06 | g | 0.06 | 0.97 |
SrCl2 x 6 H2O | 0.03 | g | 0.03 | 0.113 |
KI | 0.10 | mg | 1.0e-4 | 3.9e-4 |
Trisodium citrate | 20.00 | mg | 0.02 | 0.077 |
CaCl2 x 2 H2O | 1.50 | g | 1.5 | 10.203 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 5.5 |
Equipment needed: | Autoclave |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 27.7 | 473.991 |
Sulfur | 10 | 311.818 |
MgSO4 x 7 H2O | 7 | 28.4 |
MgCl2 x 6 H2O | 5.5 | 27.053 |
KH2PO4 | 1 | 7.348 |
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