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J354. MODIFIED SULFATE REDUCING BACTERIA MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.000 g 19.802 338.828
2 3.000 g 2.97 14.61
3 4.000 g 3.96 27.882
4 0.200 g 0.198 1.455
5 0.500 g 0.495 6.64
6 0.250 g 0.248 4.627
7 3.460 g 3.426 -
8 0.150 g 0.149 1.01
9
(BD-Difco)
0.500 g 0.495 -
10 0.166 g 0.164 2.004
11 2.000 mg 0.002 -
12 0.500 mg 5.0e-4 0.003
13 Trace vitamins 10.000 ml - -
14 0.050 mg 5.0e-5 3.1e-5
15 1.000 mg 9.9e-4 0.004
16 0.500 g 0.495 2.061
17 1000.000 ml 988.324 54860.2
Instructions:
  1. Mix ingredients, except Na2S x 9H2O, and adjust pH to 6.7 with HCl. After autoclaving under a N2 atmosphere, distribute the medium into sterile culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Neutralize 5% Na2S x 9H2O solution, and autoclave under a N2 atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution to the medium. Pressurize the inoculated vessels to 200 kPa H2-CO2 (4:1, v/v).
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 19.802 338.828
Na2SO4 3.96 27.882
PIPES 3.426 -
MgCl2 x 6H2O 2.97 14.61
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