Name: MODIFIED SULFATE REDUCING BACTERIA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J354 #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	20.000	g	19.802	338.843
MgCl₂ x 6 H₂O	3.000	g	2.97	14.61
Na₂SO₄	4.000	g	3.96	27.882
KH₂PO₄	0.200	g	0.198	1.455
KCl	0.500	g	0.495	6.641
NH₄Cl	0.250	g	0.248	4.628
PIPES	3.460	g	3.426	11.329
CaCl₂ x 2 H₂O	0.150	g	0.149	1.01
Tryptone (BD-Difco)	0.500	g	0.495	6.965
Sodium acetate	0.166	g	0.164	2.004
Na₂WO₄ x 2 H₂O	2.000	mg	0.002	0.006
Na₂SeO₄	0.500	mg	5.0e-4	0.003
Trace vitamins	10.000	ml	-	-
Vitamin B₁₂	0.050	mg	5.0e-5	3.7e-5
Resazurin	1.000	mg	9.9e-4	0.004
Na₂S x 9 H₂O	0.500	g	0.495	2.061
Distilled water	1000.000	ml	-	-
1 Mix ingredients, except Na₂S x 9H₂O, and adjust pH to 6.7 with HCl. After autoclaving under a N₂ atmosphere, distribute the medium into sterile culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H₂-CO₂ (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Neutralize 5% Na₂S x 9H₂O solution, and autoclave under a N₂ atmosphere. Prior to inoculation, add the sterile Na₂S x 9H₂O solution to the medium. Pressurize the inoculated vessels to 200 kPa H₂-CO₂ (4:1, v/v).

Trace vitamins #

1000

Solution occurs in 220 other media (first defined in Medium J197).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Biotin	2.0	mg	0.002	0.008
Folic acid	2.0	mg	0.002	0.005
Pyridoxine hydrochloride	10.0	mg	0.01	0.049
Thiamine HCl	5.0	mg	0.005	0.015
Riboflavin	5.0	mg	0.005	0.013
Nicotinic acid	5.0	mg	0.005	0.041
Calcium pantothenate	5.0	mg	0.005	0.01
Vitamin B₁₂	0.1	mg	1.0e-4	7.4e-5
p-Aminobenzoic acid	5.0	mg	0.005	0.036
Lipoic acid	5.0	mg	0.005	0.024
Distilled water	1000.0	ml	-	-

All strains for this medium:

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