Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MJ(-N) synthetic seawater | 1000.00 | ml | - | - | |
NH4Cl | 0.25 | g | 0.25 | 4.669 | |
NaHCO3 | 1.50 | g | 1.499 | 17.838 | |
Na2S2O3 x 5 H2O | 1.50 | g | 1.499 | 6.038 | |
Trace vitamins | 1.00 | ml | - | - | |
1 Mix ingredients, except NaHCO3, Na2S2O3 x 5H2O and Trace vitamins, and autoclave. Filter-sterilize 8% (w/v) NaHCO3, 10% (w/v) Na2S2O3 x 5H2O and Trace vitamins and add to the medium. Replace the gas phase with a N2-CO2-O2 (77:17:6, v/v) gas mixture, and pressurize to 200 kPa. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 30.00 | g | 29.703 | 508.264 |
K2HPO4 | 0.14 | g | 0.139 | 0.796 |
CaCl2 x 2 H2O | 0.14 | g | 0.139 | 0.943 |
MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 |
MgCl2 x 6 H2O | 4.18 | g | 4.139 | 20.357 |
KCl | 0.33 | g | 0.327 | 4.383 |
NiCl2 x 6 H2O | 0.50 | mg | 5.0e-4 | 0.004 |
Na2SeO3 x 5 H2O | 0.50 | mg | 5.0e-4 | 0.002 |
Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - |
Modified Wolin's mineral solution | 10.00 | ml | - | - |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 29.683 | 507.926 |
MgCl2 x 6 H2O | 4.134 | 20.337 |
MgSO4 x 7 H2O | 3.393 | 13.765 |
NaHCO3 | 1.499 | 17.838 |
Na2S2O3 x 5 H2O | 1.499 | 6.038 |
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