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J356. MJ BASAL MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 MJ(-N) synthetic seawater 1000.00 ml - -
2 0.25 g 0.25 4.669
3 1.50 g 1.499 17.838
4 1.50 g 1.499 -
5 Trace vitamins 1.00 ml - -
Instructions:
  1. Mix ingredients, except NaHCO3, Na2S2O3 x 5H2O and Trace vitamins, and autoclave. Filter-sterilize 8%(w/v) NaHCO3, 10%(w/v) Na2S2O3 x 5H2O and Trace vitamins and add to the medium. Replace the gas phase with a N2-CO2-O2 (77:17:6, v/v) gas mixture, and pressurize to 200 kPa.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 10 other media (first defined in medium J268 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 29.703 508.243
2 0.14 g 0.139 0.796
3 0.14 g 0.139 0.943
4 3.40 g 3.366 13.658
5 4.18 g 4.139 20.357
6 0.33 g 0.327 4.383
7 0.50 mg 5.0e-4 -
8 0.50 mg 5.0e-4 -
9 0.01 g 0.01 -
10 Trace minerals 10.00 ml - -
11 1000.00 ml 988.324 54860.2

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 29.683 507.905
MgCl2 x 6H2O 4.134 20.337
MgSO4 x 7H2O 3.393 13.764
NaHCO3 1.499 17.838
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