JCM 356 . MJ BASAL MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
MJ(-N) synthetic seawater 1000.00 ml - -
NH4Cl 0.25 g 0.25 4.669
NaHCO3 1.50 g 1.499 17.838
Na2S2O3 x 5 H2O 1.50 g 1.499 6.038
Trace vitamins 1.00 ml - -
1 Mix ingredients, except NaHCO3, Na2S2O3 x 5H2O and Trace vitamins, and autoclave. Filter-sterilize 8% (w/v) NaHCO3, 10% (w/v) Na2S2O3 x 5H2O and Trace vitamins and add to the medium. Replace the gas phase with a N2-CO2-O2 (77:17:6, v/v) gas mixture, and pressurize to 200 kPa.
Solution occurs in 12 other media (first defined in Medium J268).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.703 508.264
K2HPO4 0.14 g 0.139 0.796
CaCl2 x 2 H2O 0.14 g 0.139 0.943
MgSO4 x 7 H2O 3.40 g 3.366 13.658
MgCl2 x 6 H2O 4.18 g 4.139 20.357
KCl 0.33 g 0.327 4.383
NiCl2 x 6 H2O 0.50 mg 5.0e-4 0.004
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 0.01 -
Modified Wolin's mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 29.683 507.926
MgCl2 x 6 H2O 4.134 20.337
MgSO4 x 7 H2O 3.393 13.765
NaHCO3 1.499 17.838
Na2S2O3 x 5 H2O 1.499 6.038
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature psychrophilic 1 strain