Name: MMJ MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J360 #

Equipment needed: Anaerobic workbench Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	30.00	g	29.703	508.264
K₂HPO₄	0.14	g	0.139	0.796
CaCl₂ x 2 H₂O	1.00	g	0.99	6.735
NaNO₃	0.80	g	0.792	9.319
MgSO₄ x 7 H₂O	3.40	g	3.366	13.658
MgCl₂ x 6 H₂O	4.18	g	4.139	20.357
KCl	0.33	g	0.327	4.383
NiCl₂ x 6 H₂O	5.00	mg	0.005	0.038
Na₂SeO₃ x 5 H₂O	5.00	mg	0.005	0.019
Fe(SO₄)₃ x n H₂O	0.05	g	0.05	-
H₂WO₄	5.00	mg	0.005	0.02
Trace mineral solution	10.00	ml	-	-
NaHCO₃	1.00	g	0.99	11.786
L-Cysteine HCl x H₂O	0.50	g	0.495	-
Na₂S x 9 H₂O	0.50	g	0.495	2.061
Resazurin	1.00	mg	9.9e-4	0.004
Distilled water	1000.00	ml	-	-
1 Mix ingredients, except NaHCO₃, L-cysteine x HCl x H₂O and Na₂S x 9H₂O, adjust pH to 7.5 - 8.0, and autoclave. Separately autoclave NaHCO₃, L-cysteine x HCl x H₂O and Na₂S x 9H₂O solutions (5% each) under a N₂ atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of H₂-CO₂ (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile NaHCO₃, L-cysteine x HCl x H₂O and Na₂S x 9H₂O solutions aseptically and anaerobically. Pressurize the inoculated bottle to 200 kPa H₂-CO₂ (4:1, v/v).

Trace mineral solution #

1000

Solution occurs in 2 other media (first defined in Medium J324).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MnSO₄ x n H₂O	0.50	g	0.5	-
CoSO₄ x 7 H₂O	0.50	g	0.5	3.226
ZnSO₄ x 7 H₂O	0.18	g	0.18	0.626
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.02	g	0.02	0.077
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Archaea
Medium type:	Defined medium
Final pH:	7.8
Equipment needed:	Anaerobic workbench, Autoclave

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