J363
363 .
PY4S AGAR
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J363 #
ml
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Salt Solution I
(see Medium No. 133) |
75.00 | ml | - | - | |
|
Salt Solution II
(see Medium No. 133) |
75.00 | ml | - | - | |
|
Trypticase peptone
(BD-BBL) |
10.00 | g | 9.96 | - | |
|
Yeast extract
(BD-Difco) |
5.00 | g | 4.98 | - | |
| Glucose | 0.25 | g | 0.249 | 1.382 | |
| Cellobiose | 0.25 | g | 0.249 | 0.727 | |
| Maltose | 0.25 | g | 0.249 | 0.727 | |
|
Starch
(soluble) |
0.25 | g | 0.249 | - | |
|
Sodium resazurin
(w/v, 0.1%) |
1.00 | ml | - | - | |
|
Na2CO3
(w/v, 8%) |
2.50 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.299 | - | |
|
Agar
(BD-Difco, Bacto ) |
15.00 | g | 14.94 | - | |
| Distilled water | 850.00 | ml | - | - | |
| 1 Adjust pH to 6.7. Prepare medium anaerobically and pour into rubber stopped tubes under oxygen-free N2-CO2 (95:5). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.7 |
| Equipment needed: | Anaerobic workbench |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 10 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 14.94 | - |
| Trypticase peptone | 9.96 | - |
| Yeast extract | 4.98 | - |
| L-Cysteine HCl x H2O | 0.299 | - |
| Glucose | 0.249 | 1.382 |
| Show more | ||
