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J368. MJAIS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 29.674 507.74
2 0.90 g 0.89 5.111
3 0.70 g 0.692 5.088
4 1.00 g 0.989 6.728
5 1.25 g 1.236 23.114
6 3.40 g 3.363 13.644
7 4.18 g 4.135 20.337
8 0.33 g 0.326 4.378
9 5.00 mg 0.005 -
10 0.50 mg 4.9e-4 -
11 0.01 mg 9.9e-6 -
12 5.00 mg 0.005 0.02
13 0.02 g 0.02 0.071
14 0.02 g 0.02 -
15 Trace mineral solution 10.00 ml - -
16 Trace vitamins 1.00 ml - -
17 1.00 g 0.989 11.774
18
(powder)
10.00 g 9.891 308.464
19 0.50 g 0.495 2.059
20 1.00 mg 9.9e-4 0.004
21 1000.00 ml 987.346 54806
Instructions:
  1. Mix ingredients, except Trace vitamins, NaHCO3, sulfur and Na2S x 9H2O, adjust pH to 5.5, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.6 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the sterile Na2S x 9H2O solution. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).
Solution occurs in 2 other media (first defined in medium J368 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 0.50 g 0.5 -
3 0.50 g 0.5 -
4 0.18 g 0.18 -
5 0.01 g 0.01 -
6 0.02 g 0.02 0.077
7 0.01 g 0.01 0.162
8 0.01 g 0.01 0.041
9 0.01 g 0.01 -
10 0.01 g 0.01 0.097
11 0.01 g 0.01 0.039
12 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
NaCl 29.674 507.74
Sulfur 9.891 308.464
MgCl2 x 6H2O 4.135 20.337
MgSO4 x 7H2O 3.363 13.644
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