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JCM 368 . MJAIS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J368 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.674 507.762
K2HPO4 0.90 g 0.89 5.111
KH2PO4 0.70 g 0.692 5.088
CaCl2 x 2 H2O 1.00 g 0.989 6.728
NH4Cl 1.25 g 1.236 23.115
MgSO4 x 7 H2O 3.40 g 3.363 13.644
MgCl2 x 6 H2O 4.18 g 4.135 20.337
KCl 0.33 g 0.326 4.378
NiCl2 x 6 H2O 5.00 mg 0.005 0.038
Na2SeO3 x 5 H2O 0.50 mg 4.9e-4 0.002
Fe2(SO4)3 x n H2O 0.01 mg 9.9e-6 -
H2WO4 5.00 mg 0.005 0.02
FeSO4 x 7 H2O 0.02 g 0.02 0.071
Ferric citrate 0.02 g 0.02 0.081
Trace mineral solution 10.00 ml - -
Trace vitamins 1.00 ml - -
NaHCO3 1.00 g 0.989 11.774
Sulfur
(powder) 		10.00 g 9.891 308.425
Na2S x 9 H2O 0.50 g 0.495 2.059
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients, except Trace vitamins, NaHCO3, sulfur and Na2S x 9H2O, adjust pH to 5.5, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.6 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the sterile Na2S x 9H2O solution. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).

Trace mineral solution #

1000
Solution occurs in 2 other media (first defined in Medium J368).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x n H2O 0.50 g 0.5 -
CoSO4 x 7 H2O 0.50 g 0.5 3.226
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.02 g 0.02 0.077
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
SrCl2 x 6 H2O 0.01 g 0.01 0.038
NaBr 0.01 g 0.01 0.097
KI 0.01 g 0.01 0.039
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 29.674 507.762
Sulfur 9.891 308.425
MgCl2 x 6 H2O 4.135 20.337
MgSO4 x 7 H2O 3.363 13.644
NH4Cl 1.236 23.115
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain