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JCM 369 . MMJH MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J369 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.674 507.762
K2HPO4 0.14 g 0.138 0.795
CaCl2 x 2 H2O 1.00 g 0.989 6.728
NH4Cl 0.25 g 0.247 4.623
NaNO3 1.00 g 0.989 11.637
MgSO4 x 7 H2O 3.40 g 3.363 13.644
MgCl2 x 6 H2O 4.18 g 4.135 20.337
KCl 0.33 g 0.326 4.378
NiCl2 x 6 H2O 5.00 mg 0.005 0.038
Na2SeO3 x 5 H2O 0.50 mg 4.9e-4 0.002
Fe2(SO4)3 x n H2O 0.05 g 0.049 -
H2WO4 5.00 mg 0.005 0.02
Trace mineral solution 10.00 ml - -
Trace vitamins 1.00 ml - -
NaHCO3 1.00 g 0.989 11.774
Distilled water 1000.00 ml - -
1 Mix ingredients except Fe2(SO4)3 x xH2O, Trace vitamins and NaHCO3, adjust pH to 6.8 and autoclave. Separately autoclave 0.5% Fe2(SO4)3 x xH2O solution (in 0.1 N H2SO4), filter-sterilize Trace vitamins and 5% NaHCO3 solution and add the solutions to the medium. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2-O2 (80:20:1, v/v) gas mixture and seal with butyl rubber stoppers. Readjust pH to 6.8, if necessary. Pressurize the inoculated bottle to 200 kPa with the same gas mixture.

Trace mineral solution #

Solution occurs in 2 other media (first defined in Medium J369).
1 Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2 x H2O, 0.01 g/L NaBr and 0.01 g/L KI.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 29.674 507.762
MgCl2 x 6 H2O 4.135 20.337
MgSO4 x 7 H2O 3.363 13.644
NaHCO3 0.989 11.774
NaNO3 0.989 11.637
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain