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JCM 371 . THERMOVIBRIO AMMONIFICANS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J371 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Synthetic seawater (2 x)
(2 x) 		250.000 ml - -
NaCl 6.150 g 6.089 104.194
KH2PO4 0.500 g 0.495 3.638
CaCl2 x 2 H2O 0.375 g 0.371 2.526
(NH4)2SO4 10.000 mg 0.01 0.075
KI 0.025 mg 2.5e-5 9.5e-5
Na2WO2 x 2 H2O 0.100 mg 9.9e-5 -
NiCl2 x 6 H2O 2.000 mg 0.002 0.015
KNO3 1.000 g 0.99 9.793
MES 5.000 g 4.951 25.356
Trace minerals 10.000 ml - -
Na2S x 9 H2O 0.500 g 0.495 2.061
Resazurin 1.000 mg 9.9e-4 0.004
Distilled water 750.000 ml - -
1 Mix ingredients, except KNO3, MES and Na2S x 9H2O, bring to boil, and cool under a H2-CO2 (4:1, v/v) gas mixture. Slowly add 5% Na2S x 9H2O solution to the medium, wait for the medium to become colorless, then adjust pH to 5.5. Distribute medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers, and autoclave. Separately autoclave 10% KNO3 and 20% MES solutions (pH 5.5) under a N2 atmosphere. Prior to inoculation, add the sterile KNO3 and MES solutions. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v) gas mixture.

Synthetic seawater (2 x) #

1000
Solution occurs in 4 other media (first defined in Medium J244).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 55.40 g 55.4 947.981
MgSO4 x 7 H2O 14.00 g 14 56.801
MgCl2 x 6 H2O 11.00 g 11 54.106
KCl 1.30 g 1.3 17.438
NaBr 0.20 g 0.2 1.944
H3BO3 0.06 g 0.06 0.97
SrCl2 x 6 H2O 0.03 g 0.03 0.113
KI 0.10 mg 1.0e-4 3.9e-4
Trisodium citrate 20.00 mg 0.02 0.077
CaCl2 x 2 H2O 1.50 g 1.5 10.203
Distilled water 1000.00 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.812 339.012
MES 4.951 25.356
MgSO4 x 7 H2O 3.495 14.18
MgCl2 x 6 H2O 2.723 13.393
KNO3 0.99 9.793
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain
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