Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract
(BD-Difco) |
2.00 | g | 100 | - | |
Tryptone
(BD-Difco) |
2.00 | g | 100 | 1406.87 | |
Crotonic acid | 0.86 | g | 43 | 499.477 | |
KH2PO4 | 0.50 | g | 25 | 183.707 | |
MgCl2 x 6 H2O | 0.33 | g | 16.5 | 81.16 | |
NaCl | 0.40 | g | 20 | 342.231 | |
NH4Cl | 0.40 | g | 20 | 373.902 | |
CaCl2 x 2 H2O | 0.05 | g | 2.5 | 17.005 | |
Trace metal solution | 10.00 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
NaHCO3 | 3.50 | g | 175 | 2083.16 | |
L-Cysteine HCl x H2O | 0.50 | g | 25 | - | |
Na2S x 9 H2O | 0.50 | g | 25 | 104.088 | |
Resazurin | 1.00 | mg | 0.05 | 0.218 | |
1 Dissolve ingredients, except crotonic acid, NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, in 900 ml of distilled water and adjust pH to 7.0-7.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense 0.9 volume of the medium into suitable culture vessels under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Add 0.1 volume of a filter-sterilized crotonic acid solution (0.86 g/100 ml, pH 7.0) to the medium. Autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the solutions of crotonic acid, L-cysteine x HCl x H2O and Na2S x 9H2O to the medium. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MnSO4 x n H2O | 1.00 | g | 1 | - | |
Fe(NH4)2(SO4)2 x 6 H2O | 0.80 | g | 0.8 | - | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
ZnSO4 x 7 H2O | 0.20 | g | 0.2 | 0.696 | |
CuCl2 x 2 H2O | 0.02 | g | 0.02 | 0.117 | |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 0.02 | g | 0.02 | 0.083 | |
Na2SeO4 | 0.02 | g | 0.02 | 0.106 | |
Na2WO4 | 0.02 | g | 0.02 | 0.068 | |
Nitrilotriacetic acid | 2.00 | g | 2 | 10.464 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 6.0 with KOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.0 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 175 | 2083.16 |
Tryptone | 100 | 1406.87 |
Yeast extract | 100 | - |
Crotonic acid | 43 | 499.477 |
KH2PO4 | 25 | 183.707 |
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