MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 379 . CARNOBACTERIUM PLEISTOCAENIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J379 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 5.0 g 4.946 84.627
KH2PO4 0.3 g 0.297 2.181
KCl 0.3 g 0.297 3.98
MgSO4 x 7 H2O 0.1 g 0.099 0.401
CaSO4 x 2 H2O 12.5 mg 0.012 0.072
NH4Cl 1.0 g 0.989 18.492
NaHCO3 0.4 g 0.396 4.71
D-Glucose 5.0 g 4.946 27.451
Bacto peptone
(BD-Difco) 		1.0 g 0.989 -
Yeast extract
(BD-Difco) 		0.2 g 0.198 -
Trace vitamins 10.0 ml - -
Trace minerals solution 1.0 ml - -
Resazurin 1.0 mg 9.9e-4 0.004
Na2S x 9 H2O 0.4 g 0.396 1.647
Distilled water 1000.0 ml - -
1 Mix ingredients, except NaHCO3 and Na2S x 9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of N2 and add NaHCO3 to the medium. Anaerobically dispense the medium into culture vessels, seal with butyl rubber stoppers and autoclave. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the Na2S x 9H2O solution to the medium. Check final pH to be 7.2-7.4.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace minerals solution #

1000
Solution occurs in 5 other media (first defined in Medium J228).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
Fe(NH4)2(SO4)2 x 6 H2O 0.200 g 0.2 -
Na2SeO3 0.100 g 0.1 -
Na2MoO4 x 2 H2O 0.100 g 0.1 0.413
Na2WO4 x 2 H2O 1.000 g 1 3.032
MnSO4 x n H2O 0.100 g 0.1 -
ZnSO4 x 7 H2O 0.100 g 0.1 0.348
NiCl2 x 6 H2O 0.025 g 0.025 0.193
CuSO4 x 5 H2O 0.010 g 0.01 0.04
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals.
2 Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Anaerobic workbench, Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 4.946 84.627
D-Glucose 4.946 27.451
NH4Cl 0.989 18.492
Bacto peptone 0.989 -
NaHCO3 0.396 4.71
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain
temperature psychrophilic 1 strain