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JCM 383 . DESULFOBACTERIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J383 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 3.00 g 2.964 20.87
KH2PO4 0.20 g 0.198 1.452
NH4Cl 0.30 g 0.296 5.542
NaCl 30.00 g 29.644 507.26
MgCl2 x 6 H2O 3.00 g 2.964 14.581
KCl 0.50 g 0.494 6.627
CaCl2 x 2 H2O 0.15 g 0.148 1.008
Na2SeO3 3.00 �g - -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Trace vitamins 10.00 ml - -
Yeast extract
(BD-Difco) 		0.10 g 0.099 -
L-Sodium lactate 2.30 g 2.273 20.281
NaHCO3 2.50 g 2.47 29.407
Resazurin 1.00 mg 9.9e-4 0.004
Na2S x 9 H2O 0.40 g 0.395 1.646
Distilled water 1000.00 ml - -
1 Mix ingredients, except L-sodium lactate, NaHCO3 and Na2S x 9H2O, and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Separately, autoclave L-sodium lactate in 10 ml distilled water anaerobically and filter-sterilize 5% NaHCO3 gassed with N2-CO2 (4:1, v/v) for 20 min. Add the sterile L-sodium lactate and NaHCO3 solutions to the medium. Prior to inoculation, add an anaerobically autoclaved 5% Na2S x 9H2O solution to the medium.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 29.644 507.26
Na2SO4 2.964 20.87
MgCl2 x 6 H2O 2.964 14.581
NaHCO3 2.47 29.407
L-Sodium lactate 2.273 20.281
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain