Name: METHANOSARCINA ACETIVORANS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J385 #

Equipment needed: Anaerobic workbench Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	23.40	g	22.117	378.46
MgSO₄ x 7 H₂O	9.45	g	8.932	36.239
NH₄Cl	1.00	g	0.945	17.67
KCl	0.80	g	0.756	10.143
CaCl₂ x 2 H₂O	0.14	g	0.132	0.9
Na₂HPO₄	0.60	g	0.567	3.995
Yeast extract (BD-Difco)	1.00	g	0.945	-
Trace minerals	10.00	ml	-	-
Methanol	6.50	ml	-	-
Resazurin	1.00	mg	9.5e-4	0.004
Distilled water	1000.00	ml	-	-
1 Mix components, except methanol, and bring to a boil for 5-10 seconds. Cool down under a stream of N₂-CO₂ (80:20, v/v), add methanol to the medium and adjust pH to 7.0 with HCl. Dispense the medium into suitable culture vessels (e. g. 20 ml of the medium in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under an anaerobic gas atmosphere):
NaHCO₃ (*, 8%)	30.00	ml	-	-
L-Cysteine HCl x H₂O (5%)	6.00	ml	-	-
Na₂S x 9 H₂O (5%)	6.00	ml	-	-

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Archaea
Medium type:	Complex medium
Final pH:	7.0
Equipment needed:	Anaerobic workbench, Autoclave, Filter

Edit in Medium builder

All strains for this medium:

CSV

JSON