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JCM 388 . MV/LACTATE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J388 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.988 18.473
Na2SO4 2.0 g 1.976 13.914
Na2S2O3 x 5 H2O 1.0 g 0.988 3.981
MgSO4 x 7 H2O 1.0 g 0.988 4.009
CaCl2 x 2 H2O 0.1 g 0.099 0.672
KH2PO4 0.5 g 0.494 3.631
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
Trace vitamins 10.0 ml - -
Sodium lactate 1.0 g 0.988 8.818
Yeast extract 1.0 g 0.988 -
NaHCO3 2.0 g 1.976 23.525
Resazurin 0.5 mg 4.9e-4 0.002
Na2S x 9 H2O 75.0 mg 0.074 0.309
Distilled water 1000.0 ml - -
1 Mix ingredients, except sodium lactate, NaHCO3 and Na2S x 9H2O, bring to a boil, and cool down to room temperature under a N2 gas stream. Dispense the medium into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave sodium lactate, NaHCO3 and Na2S x 9H2O solutions (10, 8 and 0.75%, respectively) under a N2 atmosphere, and add to the medium. Check final pH to be 7.0 - 7.2.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.976 23.525
Na2SO4 1.976 13.914
NH4Cl 0.988 18.473
Sodium lactate 0.988 8.818
MgSO4 x 7 H2O 0.988 4.009
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
phyla 1 3 strains
oxygen anaerobe 3 strains
temperature thermophilic 2 strains
temperature mesophilic 1 strain