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JCM 391 . MARINE DESULFOSARCINA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J391 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.512 333.884
Na2SO4 4.00 g 3.902 27.474
MgCl2 x 6 H2O 3.00 g 2.927 14.396
KCl 0.50 g 0.488 6.543
CaCl2 x 2 H2O 0.15 g 0.146 0.995
NH4Cl 0.25 g 0.244 4.56
KH2PO4 0.20 g 0.195 1.434
Trace element solution 1.00 ml - -
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 970.00 ml - -
1 Mix components thoroughly and adjust pH 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Autoclave under the same gas mixture. After cooling, add the following solutions (filter-sterilized):
Selenite-tungstate solution 1.00 ml - -
NaHCO3
(8%) 		30.00 ml - -
Vitamin solution 1.00 ml - -
Thiamine solution 1.00 ml - -
Vitamin B12 solution 0.05 g/L 1.00 ml - -
2 Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml medium in Balch tubes) and seal with butyl rubber stoppers. Add per liter the following solution (autoclave or *filter-sterilize and store under a N2 atmosphere):
Sodium lactate
(*, 1.0 M) 		10.00 ml - -
Na2S x 9 H2O
(5%) 		10.00 ml - -
3 Readjust pH to 7.0 - 7.3, if necessary.
4 Comment: For strain JCM 31729, replace sodium lactate with final 10 mM sodium fumarate and 0.01% yeast extract.

Trace element solution #

1000
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Vitamin solution #

100
Solution occurs in 14 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 4 mg 0.04 0.292
Biotin 1 mg 0.01 0.041
Nicotinic acid 10 mg 0.1 0.812
DL-Calcium pantothenate 5 mg 0.05 0.105
Pyridoxine hydrochloride 10 mg 0.1 -
Sodium phosphate buffer
(10 mM, pH 7.1) 		100 ml - -

Thiamine solution #

100
Solution occurs in 10 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine HCl 10 mg 0.1 -
Sodium phosphate buffer
(25 mM, pH 3.4) 		100 ml - -

Vitamin B12 solution 0.05 g/L #

100
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.512 333.884
Na2SO4 3.902 27.474
MgCl2 x 6 H2O 2.927 14.396
KCl 0.488 6.543
NH4Cl 0.244 4.56
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains