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JCM 395 . DESULFOBACTERIUM CETONICUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J395 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 2.80 g 2.794 19.673
KH2PO4 0.70 g 0.699 5.134
NH4Cl 0.30 g 0.299 5.597
NaCl 10.00 g 9.98 170.774
MgCl2 x 6 H2O 1.70 g 1.697 8.345
CaCl2 x 2 H2O 0.05 g 0.05 0.339
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Sodium butyrate 1.20 g 1.198 10.878
NaHCO3 2.00 g 1.996 23.76
Resazurin 1.00 mg 1.0e-3 0.004
Na2S x 9 H2O 0.30 g 0.299 1.247
Distilled water 1000.00 ml - -
1 Mix ingredients, except sodium butyrate, NaHCO3 and Na2S x 9H2O, and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Separately, autoclave sodium butyrate in 10 ml distilled water under a N2 atmosphere. Filter-sterilize 5% NaHCO3 and store under a N2-CO2 (4:1, v/v) atmosphere. Add the sterile sodium butyrate and NaHCO3 solutions to the medium. Prior to inoculation, add a 5% Na2S x 9H2O solution (anaerobically autoclaved) to the medium. Check final pH of the medium to be 7.2 - 7.4.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 9.98 170.774
Na2SO4 2.794 19.673
NaHCO3 1.996 23.76
MgCl2 x 6 H2O 1.697 8.345
Sodium butyrate 1.198 10.878
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain