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JCM 397 . TD3 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J397 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 4.00 g 3.876 27.288
KH2PO4 0.20 g 0.194 1.424
NH4Cl 0.25 g 0.242 4.529
NaCl 20.00 g 19.38 331.62
MgCl2 x 6 H2O 9.80 g 9.496 46.709
KCl 0.50 g 0.484 6.499
CaCl2 x 2 H2O 0.10 g 0.097 0.659
Na2SeO3 6.00 �g - -
Na2WO4 x 2 H2O 8.00 �g - -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Trace vitamins 20.00 ml - -
Vitamin B12 0.10 mg 9.7e-5 7.1e-5
Capronic acid 0.50 g 0.484 4.171
Fatty acid mixture 10.00 ml - -
NaHCO3 2.50 g 2.422 28.837
Resazurin 0.50 mg 4.8e-4 0.002
Na2S x 9 H2O 0.40 g 0.388 1.614
Distilled water 1000.00 ml - -
1 Mix ingredients, except capronic acid, Trace vitamins, vitamin B12, NaHCO3, and Na2 x 9H2O, and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Add capronic acid to 10 ml distilled water and adjust pH to 7.5 with NaOH. Filter-sterilize the capronic acid solution, Fatty acid mixture and Trace vitamins with vitamin B12, and store under a N2 atmosphere. Filter-sterilize 5% NaHCO3 solution and store under a N2-CO2 (4:1, v/v) atmosphere. Add the capronic acid solution, Fatty acid mixture, Trace vitamins and 5% NaHCO3 solution to the medium. Prior to inoculation, add a 5% Na2S x 9H2O solution (anaerobically autoclaved) to the medium. Adjust pH of the medium to 6.7 - 6.9, if necessary.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Fatty acid mixture #

20
Solution occurs in 5 other media (first defined in Medium J266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Valeric acid 0.5 g 25 244.786
Isovaleric acid 0.5 g 25 244.786
alpha-Methylbutyric acid 0.5 g 25 244.786
Isobutyric acid 0.5 g 25 283.736
Distilled water 20.0 ml - -
1 Adjust pH to 7.5 with concentrated NaOH solution.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.38 331.62
MgCl2 x 6 H2O 9.496 46.709
Na2SO4 3.876 27.288
NaHCO3 2.422 28.837
KCl 0.484 6.499
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain