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JCM 399 . DESULFOVIBRIO MAGNETICUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J399 #

ml
Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.20 g 0.197 1.132
NH4Cl 0.06 g 0.059 1.106
Fe(III) quinate solution 2.00 ml - -
Trace minerals 4.00 ml - -
Trace vitamins 8.00 ml - -
Sodium fumarate 0.58 g 0.572 3.574
Sodium pyruvate 0.44 g 0.434 3.943
L-Cysteine HCl x H2O 0.05 g 0.049 -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0. Prepare medium anaerobically under a N2 gas atmosphere.

Fe(III) quinate solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 0.45 g 4.5 16.648
Quinic acid 0.19 g 1.9 9.887
Distilled water 100.00 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Anaerobic workbench
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium fumarate 0.572 3.574
Sodium pyruvate 0.434 3.943
K2HPO4 0.197 1.132
NH4Cl 0.059 1.106
L-Cysteine HCl x H2O 0.049 -
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