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J399. DESULFOVIBRIO MAGNETICUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.197 1.132
2 0.06 g 0.059 1.106
3 Fe(III) quinate solution 2.00 ml - -
4 Trace minerals 4.00 ml - -
5 Trace vitamins 8.00 ml - -
6 0.58 g 0.572 -
7 0.44 g 0.434 -
8 0.05 g 0.049 0.281
9 1000.00 ml 984.425 54643.8
Instructions:
  1. Adjust pH to 7.0. Prepare medium anaerobically under a N2 gas atmosphere.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.45 g 4.5 -
2 0.19 g 1.9 -
3 100.00 ml 998.207 55408.8
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Sodium fumarate 0.572 -
Sodium pyruvate 0.434 -
K2HPO4 0.197 1.132
NH4Cl 0.059 1.106
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