399 .
DESULFOVIBRIO MAGNETICUS MEDIUM
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Equipment needed:
Anaerobic workbench
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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K2HPO4 | 0.20 | g | ||
|
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NH4Cl | 0.06 | g | ||
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Fe(III) quinate solution | 2.00 | ml | ||
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Trace minerals | 4.00 | ml | ||
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Trace vitamins | 8.00 | ml | ||
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Sodium fumarate | 0.58 | g | ||
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Sodium pyruvate | 0.44 | g | ||
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L-Cysteine HCl x H2O | 0.05 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.0. Prepare medium anaerobically under a N2 gas atmosphere. | |||||
Fe(III) quinate solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeCl3 x 6 H2O | 0.45 | g |
|
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Quinic acid | 0.19 | g |
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Distilled water | 100.00 | ml |
Trace minerals #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
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MgSO4 x 7 H2O | 3.00 | g | ||
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MnSO4 x n H2O | 0.50 | g | ||
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NaCl | 1.00 | g | ||
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FeSO4 x 7 H2O | 0.10 | g | ||
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CoSO4 x 7 H2O | 0.10 | g | ||
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CaCl2 x 2 H2O | 0.10 | g | ||
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ZnSO4 x 7 H2O | 0.10 | g | ||
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CuSO4 x 5 H2O | 0.01 | g | ||
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AlK(SO4)2 | 0.01 | g | ||
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H3BO3 | 0.01 | g | ||
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Na2MoO4 x 2 H2O | 0.01 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
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Folic acid | 2.0 | mg |
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Pyridoxine hydrochloride | 10.0 | mg |
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
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Calcium pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
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p-Aminobenzoic acid | 5.0 | mg |
|
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Lipoic acid | 5.0 | mg |
|
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Distilled water | 1000.0 | ml |