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JCM 401 . PROPIONIVIBRIO MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 15.385 113.051
NH4Cl 0.25 g 19.231 359.521
NaCl 1.00 g 76.923 1316.27
MgCl2 x 6 H2O 0.40 g 30.769 151.347
KCl 0.50 g 38.462 515.916
CaCl2 x 2 H2O 0.15 g 11.539 78.485
Trace minerals 1.00 ml - -
Trace vitamins 10.00 ml - -
Quinic acid
(5 mM)
1.00 ml - -
Resazurin 1.00 mg 0.077 0.336
NaHCO3 2.50 g 192.308 2289.19
2,3-butanediol 0.90 g 69.231 768.206
Na2S x 9 H2O 0.36 g 27.692 115.297
Distilled water 1.00 ml - -
1 Adjust pH to 7.2. Prepare medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture.
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Anaerobic workbench

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 192.308 2289.19
NaCl 77 1317.59
2,3-butanediol 69.231 768.206
KCl 38.462 515.916
MgCl2 x 6 H2O 30.769 151.347
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