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J403. ACETOBACTERIUM SUBMARINUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(Sigma)
30.00 g 29.732 -
2
(BD-Difco)
0.10 g 0.099 -
3 Trace element solution 1.00 ml - -
4 Vitamin solution 1.00 ml - -
5 Thiamine solution 1.00 ml - -
6 Vitamin B12 solution 1.00 ml - -
7 30.00 mg 0.03 -
8 0.50 mg 5.0e-4 0.003
9 1.00 g 0.991 12.081
10 2.00 g 1.982 63.823
11 5.00 g 4.955 72.866
12 5.00 ml 3.915 122.176
13 0.20 g 0.198 1.457
14 0.27 g 0.268 5.003
15 0.50 mg 5.0e-4 0.002
16 1.26 g 1.249 14.865
17 0.50 g 0.496 2.063
18 1000.00 ml 989.303 54914.6
Instructions:
  1. Mix ingredients except methanol, NaHCO3 and Na2S x 9H2O, bring to a boil and cool down under a stream of N2 gas. Add methanol to the medium and distribute into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers, and autoclave. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Filer-sterilize 8% NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. Before inoculation, add the sterile NaHCO3 and Na2S x 9H2O solutions to the medium, and readjust pH to 7.0, if necessary. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
Solution occurs in 14 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.04 0.292
2 1 mg 0.01 0.041
3 10 mg 0.1 0.812
4 5 mg 0.05 -
5 10 mg 0.1 -
6
(10 mM, pH 7.1)
100 ml - -
Solution occurs in 10 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 mg 0.1 -
2
(25 mM, pH 3.4)
100 ml - -
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8
Solution occurs in 5 other media (first defined in medium J301 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 12.80 g - -
2 0.42 g - -
3 0.01 g - -
4 0.10 g - -
5 0.17 g - -
6 0.02 g - -
7 0.21 g - -
8 0.01 g - -
Instructions:
  1. Dissolve nitrilotriacetic acid in 800 ml distilled water and adjust pH to 6.5 with NaOH. Then proceed to add the minerals and bring volume to 1.0 L.

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 990.292 54969.5
Sea salts 29.732 -
Sodium formate 4.955 72.866
Methanol 3.915 122.176
Methylamine 1.982 63.823
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