J403
403 .
ACETOBACTERIUM SUBMARINUS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J403 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(Sigma) |
30.00 | g | 29.732 | - | |
|
Yeast extract
(BD-Difco) |
0.10 | g | 0.099 | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Vitamin solution | 1.00 | ml | - | - | |
| Thiamine solution | 1.00 | ml | - | - | |
| Vitamin B12 solution 0.05 g/L | 1.00 | ml | - | - | |
| Na2WO4 x 2 H2O | 30.00 | mg | 0.03 | 0.09 | |
| Na2SeO4 | 0.50 | mg | 5.0e-4 | 0.003 | |
| Sodium acetate | 1.00 | g | 0.991 | 12.082 | |
| Methylamine | 2.00 | g | 1.982 | 63.823 | |
| Sodium formate | 5.00 | g | 4.955 | 72.866 | |
| Methanol | 5.00 | ml | - | - | |
| KH2PO4 | 0.20 | g | 0.198 | 1.457 | |
| NH4Cl | 0.27 | g | 0.268 | 5.003 | |
| Resazurin | 0.50 | mg | 5.0e-4 | 0.002 | |
| NaHCO3 | 1.26 | g | 1.249 | 14.865 | |
| Na2S x 9 H2O | 0.50 | g | 0.496 | 2.063 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients except methanol, NaHCO3 and Na2S x 9H2O, bring to a boil and cool down under a stream of N2 gas. Add methanol to the medium and distribute into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers, and autoclave. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Filer-sterilize 8% NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. Before inoculation, add the sterile NaHCO3 and Na2S x 9H2O solutions to the medium, and readjust pH to 7.0, if necessary. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v). | |||||
Solution occurs in 14
other media (first defined in Medium J403).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| p-Aminobenzoic acid | 4 | mg | 0.04 | 0.292 |
| Biotin | 1 | mg | 0.01 | 0.041 |
| Nicotinic acid | 10 | mg | 0.1 | 0.812 |
| DL-Calcium pantothenate | 5 | mg | 0.05 | 0.105 |
| Pyridoxine hydrochloride | 10 | mg | 0.1 | - |
|
Sodium phosphate buffer
(10 mM, pH 7.1) |
100 | ml | - | - |
Solution occurs in 10
other media (first defined in Medium J403).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Thiamine HCl | 10 | mg | 0.1 | - |
|
Sodium phosphate buffer
(25 mM, pH 3.4) |
100 | ml | - | - |
Solution occurs in 18
other media (first defined in Medium J403).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Vitamin B12 | 5 | mg | 0.05 | 0.037 |
| Distilled water | 100 | ml | - | - |
Solution occurs in 5
other media (first defined in Medium J301).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 12.80 | g | - | - | |
| FeSO4 x 7 H2O | 0.42 | g | - | - | |
| H3BO3 | 0.01 | g | - | - | |
| MnCl2 x 4 H2O | 0.10 | g | - | - | |
| CoCl2 x 6 H2O | 0.17 | g | - | - | |
| CuCl2 x 2 H2O | 0.02 | g | - | - | |
| ZnSO4 x 7 H2O | 0.21 | g | - | - | |
| Na2MoO4 x 2 H2O | 0.01 | g | - | - | |
| 1 Dissolve nitrilotriacetic acid in 800 ml distilled water and adjust pH to 6.5 with NaOH. Then proceed to add the minerals and bring volume to 1.0 L. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |