J411
411 .
Fe(III)-REDUCER MARINE ENRICHMENT MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J411 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 19.000 | g | 950 | 16256 | |
| MgCl2 x 6 H2O | 9.000 | g | 450 | 2213.45 | |
| MgSO4 x 7 H2O | 0.150 | g | 7.5 | 30.429 | |
| CaCl2 x 2 H2O | 0.300 | g | 15 | 102.03 | |
| KCl | 0.500 | g | 25 | 335.345 | |
| KH2PO4 | 0.420 | g | 21 | 154.314 | |
| (NH4)2SO4 | 0.100 | g | 5 | 37.839 | |
| NaBr | 0.050 | g | 2.5 | 24.298 | |
| SrCl2 x 6 H2O | 0.020 | g | 1 | 3.751 | |
|
Yeast extract
(BD-Difco) |
0.100 | g | 5 | - | |
| NaHCO3 | 2.500 | g | 125 | 1487.97 | |
| Na2SeO4 | 0.020 | g | 1 | 5.292 | |
| Poorly crystalline iron(III) oxide | 100.000 | mM | - | - | |
| Trace vitamins | 10.000 | ml | - | - | |
| Trace mineral solution | 10.000 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.044 | g | 2.2 | - | |
| FeCl2 x 4 H2O | 0.260 | g | 13 | 65.388 | |
| 1 Add components except L-cysteine x HCl x H2O and FeCl2 x 4H2O to distilled water, bring volume to 1.0 L and adjust pH to 7.0. Distribute the medium into appropriate culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, sparge with the same gas mixture for 10 - 15 min, seal with butyl rubber stoppers and autoclave. Separately autoclave concentrated (100- or 200-fold) L-cysteine x HCl x H2O and FeCl2 x 4H2O solutions under a N2 gas atmosphere. Prior to inoculation, add the sterile L-cysteine x HCl x H2O and FeCl2 x 4H2O solutions. Pressurize the inoculated vessels to 100 kPa H2-CO2 (4:1, v/v) and cultivate without shaking. | |||||
Solution occurs in 3
other media (first defined in Medium J411).
Equipment needed: Centrifuge
| 1 Dissolve 108 g FeCl3 in 1.0 L distilled water. Adjust pH to 7.0 with 10 N NaOH. Allow the suspension to sit for 30 min, then readjust pH to 7.0. Centrifuge the suspension at 5,000 x g for 20 min and pour off supernatant. Wash the precipitate with distilled water by resuspending in distilled water followed by the centrifugation. Repeat the washing at least 5 times, then, resuspend in 1.0 L distilled water. Use the Fe(III) suspension as 0.67 M solution. More precisely, check the concentration of Cl- to be less than 1 mM and determine the concentration of Fe(III) by using hydroxylamine as described by Lovley and Phillips (Appl. Environ. Microbiol. 54, 751-757, 1986). |
Solution occurs in 4
other media (first defined in Medium J411).
| 1 Use Trace minerals of Medium No. 151 supplemented with 0.025 g (final) Na2MoO4 x 2H2O, 0.024 g NiCl2 x 6H2O and 0.025 g Na2WO4 x 2H2O (per liter). | |||||
| 2 Comment: For strain JCM 12378, inoculate with a 10% inoculum. |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Centrifuge |
| Edit in Medium builder | |