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JCM 416 . MODIFIED MARINE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J416 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 19.000 g 18.628 318.745
MgCl2 x 6 H2O 9.000 g 8.824 43.401
MgSO4 x 7 H2O 0.150 g 0.147 0.597
CaCl2 x 2 H2O 0.300 g 0.294 2.001
KCl 0.500 g 0.49 6.575
KH2PO4 0.420 g 0.412 3.026
(NH4)2SO4 0.100 g 0.098 0.742
NaBr 0.050 g 0.049 0.476
SrCl2 x 6 H2O 0.020 g 0.02 0.074
Yeast extract
(BD-Difco) 		0.200 g 0.196 -
Na2SeO4 0.020 g 0.02 0.104
DL-Sodium malate 1.800 g 1.765 9.911
KNO3 1.000 g 0.98 9.697
Trace vitamins 10.000 ml - -
Trace mineral solution 10.000 ml - -
L-Cysteine HCl x H2O 0.088 g 0.086 -
Distilled water 1000.000 ml - -
1 Mix components, except L-cysteine x HCl x H2O, and adjust pH to 6.5. Distribute the medium into appropriate culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave concentrated (100- or 200-fold) L-cysteine x HCl x H2O solution under a N2 atmosphere. Prior to inoculation, add the sterile L-cysteine x HCl x H2O solution. Pressurize the inoculated vessels to 100 kPa N2.
2 Comment: For strain JCM 12418, inoculate with a 10% inoculum.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace mineral solution #

Solution occurs in 4 other media (first defined in Medium J411).
1 Use Trace minerals of Medium No. 151 supplemented with 0.025 g (final) Na2MoO4 x 2H2O, 0.024 g NiCl2 x 6H2O and 0.025 g Na2WO4 x 2H2O (per liter).
2 Comment: For strain JCM 12378, inoculate with a 10% inoculum.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 18.628 318.745
MgCl2 x 6 H2O 8.824 43.401
DL-Sodium malate 1.765 9.911
KNO3 0.98 9.697
KCl 0.49 6.575
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain