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JCM 417 . MJYTFSH MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J417 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.782 338.508
K2HPO4 0.90 g 0.89 5.111
KH2PO4 0.70 g 0.692 5.088
CaCl2 x 2 H2O 1.00 g 0.989 6.728
NH4Cl 1.25 g 1.236 23.115
MgSO4 x 7 H2O 3.40 g 3.363 13.644
MgCl2 x 6 H2O 4.18 g 4.135 20.337
KCl 0.33 g 0.326 4.378
NiCl2 x 6 H2O 5.00 mg 0.005 0.038
Na2SeO3 x 5 H2O 5.00 mg 0.005 0.019
Fe2(SO4)3 x n H2O 0.01 g 0.01 -
H2WO4 5.00 mg 0.005 0.02
FeSO4 x 7 H2O 0.02 g 0.02 0.071
Yeast extract
(BD-Difco) 		1.00 g 0.989 -
Tryptone
(BD-Difco) 		1.00 g 0.989 13.916
Sodium formate 0.20 g 0.198 2.909
Trace mineral solution 10.00 ml - -
Trace vitamins 1.00 ml - -
NaHCO3 2.00 g 1.978 23.549
Sulfur
(powder) 		10.00 g 9.891 308.425
Na2S x 9 H2O 0.50 g 0.495 2.059
Distilled water 1000.00 ml - -
1 Mix ingredients, except Trace vitamins, NaHCO3 and sulfur, adjust pH to 5.5, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sterilized sulfur under a stream of H2-CO2 (4:1, v/v) gas mixture, sparge for 5 min with the same gas mixture, and seal with butyl rubber stoppers. Prior to inoculation, add the sterilized Na2S x 9H2O solution to the medium, and pressurize to 200 kPa H2-CO2 (4:1, v/v). Readjust pH to 5.5, if necessary.

Trace mineral solution #

Solution occurs in 2 other media (first defined in Medium J369).
1 Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2 x H2O, 0.01 g/L NaBr and 0.01 g/L KI.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.782 338.508
Sulfur 9.891 308.425
MgCl2 x 6 H2O 4.135 20.337
MgSO4 x 7 H2O 3.363 13.644
NaHCO3 1.978 23.549
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain