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JCM 419 . INORGANIC MINERAL MEDIUM WITH THIOSULFATE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J419 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 3.000 g 2.994 51.232
K2HPO4 0.014 g 0.014 0.08
CaCl2 x 2 H2O 0.080 g 0.08 0.543
MgSO4 x 7 H2O 0.340 g 0.339 1.377
MgCl2 x 6 H2O 0.418 g 0.417 2.052
KCl 0.330 g 0.329 4.418
NH4Cl 0.500 g 0.499 9.329
Na2S2O3 x 5 H2O 1.000 g 0.998 4.021
Na2SiO3 x 9 H2O 0.800 g 0.798 -
Fe2(SO4)3 x n H2O 2.000 mg 0.002 -
FeSO4 x 7 H2O 0.100 mg 1.0e-4 3.6e-4
NiCl2 x 6 H2O 0.050 mg 5.0e-5 3.9e-4
Na2SeO3 x 5 H2O 0.050 mg 5.0e-5 1.9e-4
Trace mineral solution 1.000 ml - -
Trace vitamins 1.000 ml - -
NaHCO3 0.500 g 0.499 5.94
Distilled water 1000.000 ml - -
1 Mix ingredients, except Trace vitamins and NaHCO3, and adjust pH to 7.0. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Finally add O2 gas to make 5% (v/v) in the gas phase.

Trace mineral solution #

1000
Solution occurs in 2 other media (first defined in Medium J324).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x n H2O 0.50 g 0.5 -
CoSO4 x 7 H2O 0.50 g 0.5 3.226
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.02 g 0.02 0.077
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 2.994 51.232
Na2S2O3 x 5 H2O 0.998 4.021
Na2SiO3 x 9 H2O 0.798 -
NH4Cl 0.499 9.329
NaHCO3 0.499 5.94
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature thermophilic 1 strain