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J419. INORGANIC MINERAL MEDIUM WITH THIOSULFATE

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.000 g 2.994 51.23
2 0.014 g 0.014 0.08
3 0.080 g 0.08 0.543
4 0.340 g 0.339 1.377
5 0.418 g 0.417 2.052
6 0.330 g 0.329 4.418
7 0.500 g 0.499 9.329
8 1.000 g 0.998 -
9 0.800 g 0.798 -
10 2.000 mg 0.002 -
11 0.100 mg 1.0e-4 3.6e-4
12 0.050 mg 5.0e-5 -
13 0.050 mg 5.0e-5 -
14 Trace mineral solution 1.000 ml - -
15 Trace vitamins 1.000 ml - -
16 0.500 g 0.499 5.94
17 1000.000 ml 996.215 55298.2
Instructions:
  1. Mix ingredients, except Trace vitamins and NaHCO3, and adjust pH to 7.0. Distribute the medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Finally add O2 gas to make 5%(v/v) in the gas phase.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 2 other media (first defined in medium J324 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 0.50 g 0.5 -
3 0.50 g 0.5 -
4 0.18 g 0.18 -
5 0.01 g 0.01 -
6 0.02 g 0.02 0.077
7 0.01 g 0.01 0.162
8 0.01 g 0.01 0.041
9 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 2.994 51.23
Na2S2O3 x 5H2O 0.998 -
Na2SiO3 x 9H2O 0.798 -
NH4Cl 0.499 9.329
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