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JCM 420 . MJAIS/MD MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J420 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.674 507.762
K2HPO4 0.09 g 0.089 0.511
KH2PO4 0.07 g 0.069 0.509
CaCl2 x 2 H2O 0.80 g 0.791 5.382
NH4Cl 1.25 g 1.236 23.115
MgSO4 x 7 H2O 3.40 g 3.363 13.644
MgCl2 x 6 H2O 4.18 g 4.135 20.337
KCl 0.33 g 0.326 4.378
NiCl2 x 6 H2O 10.00 mg 0.01 0.076
Na2SeO3 x 5 H2O 10.00 mg 0.01 0.038
Na2WO4 x 2 H2O 10.00 mg 0.01 0.03
Fe(NH4)2(SO4)2 x 6 H2O 10.00 mg 0.01 -
FeSO4 x 7 H2O 20.00 mg 0.02 0.071
Trace mineral solution 10.00 ml - -
Trace vitamins 1.00 ml - -
NaHCO3 2.00 g 1.978 23.549
Sulfur
(powder) 		30.00 g 29.674 925.276
Na2S x 9 H2O 0.50 g 0.495 2.059
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients, except Trace vitamins, NaHCO3, sulfur and Na2S x 9H2O, adjust pH to 6.0, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.6 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the sterile Na2S x 9H2O solution. Pressurize the inoculated bottle to 300 kPa H2-CO2 (4:1, v/v).

Trace mineral solution #

Solution occurs in 2 other media (first defined in Medium J369).
1 Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2 x H2O, 0.01 g/L NaBr and 0.01 g/L KI.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 29.674 925.276
NaCl 29.674 507.762
MgCl2 x 6 H2O 4.135 20.337
MgSO4 x 7 H2O 3.363 13.644
NaHCO3 1.978 23.549
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain