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J425. A1 MARINE MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Poorly crystalline iron(III) oxide 100.00 mM - -
2 20.00 g 400 6844.33
3 0.77 g 15.4 206.57
4 0.25 g 5 93.473
5 0.10 g 2 14.697
6 0.20 g 4 16.229
7 Trace vitamins 10.00 ml - -
8 Trace mineral solution 10.00 ml - -
9 Acetate solution 10.00 ml - -
10 APM salts solution 20.00 ml - -
Instructions:
  1. Add components except acetate and APM salts solutions to distilled water, bring volume to 1.0 L and adjust pH to 6.8. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, sparge with the same gas mixture for 10 - 15 min, seal with butyl rubber stoppers and autoclave. Separately autoclave acetate and APM salts solutions under a N2 gas atmosphere and add to the medium anaerobically.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 41 g 41 499.794
2 1000 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 21.20 g 21.2 104.279
2 3.04 g 3.04 20.679
3 1000.00 ml 998.207 55408.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 4 other media (first defined in medium J411 )
Instructions:
  1. Use Trace minerals of Medium No. 151 supplemented with 0.025 g (final) Na2MoO4 x 2H2O, 0.024 g NiCl2 x 6H2O and 0.025 g Na2WO4 x 2H2O (per liter).
  2. Comment: For strain JCM 12378, inoculate with a 10% inoculum.
Solution occurs in 3 other media (first defined in medium J411 )
Instructions:
  1. Dissolve 108 g FeCl3 in 1.0 L distilled water. Adjust pH to 7.0 with 10 N NaOH. Allow the suspension to sit for 30 min, then readjust pH to 7.0. Centrifuge the suspension at 5,000 x g for 20 min and pour off supernatant. Wash the precipitate with distilled water by resuspending in distilled water followed by the centrifugation. Repeat the washing at least 5 times, then, resuspend in 1.0 L distilled water. Use the Fe(III) suspension as 0.67 M solution. More precisely, check the concentration of Cl- to be less than 1 mM and determine the concentration of Fe(III) by using hydroxylamine as described by Lovley and Phillips (Appl. Environ. Microbiol. 54, 751-757, 1986).

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 798.566 44327
NaCl 400 6844.33
KCl 15.4 206.57
MgCl2 x 6H2O 8.48 41.712
Sodium acetate 8.2 99.959
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